CC BY NC SAYang, KunPark, Chae G.Bulgheresi, SilviaJiang, LingyuWang, ShaogangSkurnik, MikaelCheong, CheolhoZhang, ShushengZhang, PeiHe, YingxiaHuang, HongpingDing, HonghuiWu, YipingZhang, LinLi, AnyiXia, LianxuBartra, Sara S.Plano, Gregory V.Klena, John D.Chen, Tie2025-06-192025-06-192015-04-210818-96411440-171110.1038/icb.2015.46https://ror.circle-u.eu/handle/123456789/1244803<jats:p><jats:italic>Yersinia pestis</jats:italic> is a Gram‐negative bacterium that causes plague. After <jats:italic>Y. pestis</jats:italic> overcomes the skin barrier, it encounters antigen‐presenting cells (APCs), such as Langerhans and dendritic cells. They transport the bacteria from the skin to the lymph nodes. However, the molecular mechanisms involved in bacterial transmission are unclear. Langerhans cells (LCs) express Langerin (CD207), a calcium‐dependent (C‐type) lectin. Furthermore, <jats:italic>Y. pestis</jats:italic> possesses exposed core oligosaccharides. In this study, we show that <jats:italic>Y. pestis</jats:italic> invades LCs and Langerin‐expressing transfectants. However, when the bacterial core oligosaccharides are shielded or truncated, <jats:italic>Y. pestis</jats:italic> propensity to invade Langerhans and Langerin‐expressing cells decreases. Moreover, the interaction of <jats:italic>Y. pestis</jats:italic> with Langerin‐expressing transfectants is inhibited by purified Langerin, a DC‐SIGN (DC‐specific intercellular adhesion molecule 3 grabbing nonintegrin)‐like molecule, an anti‐CD207 antibody, purified core oligosaccharides and several oligosaccharides. Furthermore, covering core oligosaccharides reduces the mortality associated with murine infection by adversely affecting the transmission of <jats:italic>Y. pestis</jats:italic> to lymph nodes. These results demonstrate that direct interaction of core oligosaccharides with Langerin facilitates the invasion of LCs by <jats:italic>Y. pestis</jats:italic>. Therefore, Langerin‐mediated binding of <jats:italic>Y. pestis</jats:italic> to APCs may promote its dissemination and infection.</jats:p>OPENDendritic Cells/metabolismLangerhans Cells/metabolismYersinia pestis/metabolismC-Type/immunology*Cell Surface/immunologyBacterial AdhesionO-ANTIGENMiceCORE LIPOPOLYSACCHARIDEAntigen-Presenting Cells/microbiologyLectinsCricetinaeReceptorsO Antigens/metabolismCells, CulturedO Antigens/immunology106022 MikrobiologieCulturedProtein Binding/immunologyHIV-1 TRANSMISSIONCD/immunology*CD/metabolismO AntigensBacterial Adhesion/immunologyFlow CytometryHost-Pathogen Interactions/immunologyPlague/microbiologyPlague/immunologyHost-Pathogen Interactions106022 MicrobiologyNEISSERIA-GONORRHOEAEProtein Binding570ESCHERICHIA-COLI K-12Langerhans Cells/immunologyCellsCell Surface/metabolism610Antigen-Presenting CellsRFB GENE-CLUSTERCHO CellsDENDRITIC CELLSCricetulusPhagocytosisAntigens, CDAnimalsHumansLectins, C-TypeAntigensPLASMINOGEN-ACTIVATORMannose-Binding Lectins/metabolismC-Type/metabolismPlagueYersinia pestis/immunology*DC-SIGN CD209Phagocytosis/immunology*Original ArticlesDendritic CellsSurvival AnalysisBiomedicineMannose-Binding LectinsDendritic Cells/immunologyMannose-Binding Lectins/immunology*Langerhans CellsMONOCLONAL-ANTIBODIESAntigen-Presenting Cells/immunology*Yersinia pestis/physiologypublication3. Good healthdoi_dedup___:e39fb4e4290c273a242560845ca763bePMC46127762582914110138/232575