c_abf2Katarina StevanovićKristina AtlagićNikola UnkovićTijana Cvetić AntićTanja PajićJovana LukičićKatarina KruščićMiroslav ŽivićNataša V. Todorović2025-12-312025-12-312025-07-182309-608X10.3390/jof11070535https://ror.circle-u.eu/handle/123456789/1715318The potential of Trichoderma fungi as biocontrol agents has not yet been fully explored, as there is a large repertoire of inter- and intra-species variation in their phytopathogenic antagonistic effects due to different adaptations of individual Trichoderma strains. In the present study, we investigated the biocontrol efficacy of eight native isolates of Trichoderma spp. against the soilborne phytopathogens Sclerotinia sclerotiorum and Rhizoctonia solani and a representative of the Mucoromycota, Phycomyces blakesleeanus. An in vitro dual culture test showed a complete (100%) inhibition of S. sclerotiorum and P. blakesleeanus by each tested Trichoderma strain and a high (80–100%) inhibition of R. solani. The crude chloroform extracts, whose peptide contents were confirmed by thin-layer chromatography, caused a concentration-dependent reduction in the growth of the target fungi, with inhibition comparable to the effect of the peptaibol standard alamethicin. Despite the differences between fungi from the phyla Basidiomycota, Ascomycota, and Mucoromycota, their inhibition by alamethicin followed the same dose–response dependence. The growth inhibition of P. blakesleeanus induced by Trichoderma extracts was characterized by a significantly increased activity of antioxidative defense enzymes. Both variants of biocontrol agents, the native strains of Trichoderma spp. and their extracts, are efficient in controlling fungal growth and should be considered for the development of new potent bioformulations applicable in agriculture.OPENPhycomyces blakesleeanusTrichodermaalamethicinQH301-705.5Rhizoctonia solaniantioxidative defense<i>Trichoderma</i>Article<i>Rhizoctonia solani</i>growth inhibition<i>Phycomyces blakesleeanus</i>Sclerotinia sclerotiorum<i>Sclerotinia sclerotiorum</i>biocontrolpeptaibolBiology (General)publication