Ultrastructural and biochemical observations on interphase nuclei isolated from chicken erythrocytes
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The Company of Biologists
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Abstract
ABSTRACTAdult hen erythrocyte nuclei are isolated from cells or haemolysed in situ by acting on the plasma membrane with rotating knives or with non-ionic detergents. When the isolation medium contains magnesium 10ns (1 mM), sucrose (0·4 M) and Tris buffer (0· 01 M, pH 7· 5) called SMTOG (see text),...
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Keywords
Cell Nucleus , Cytoplasm , Erythrocytes , Staining and Labeling , Blood Proteins , DNA , Phosphotungstic Acid , Hemolysis , Chromatin , Polyethylene Glycols , Histones , Models, Structural , Hemoglobins , Microscopy, Electron , Spectrophotometry , Animals , Electrophoresis, Polyacrylamide Gel , Magnesium , Chickens , Cell Division